In vitro plant culture

The invention: Method for propagating plants in artificial media
that has revolutionized agriculture.
The people behind the invention:
Georges Michel Morel (1916-1973), a French physiologist
Philip Cleaver White (1913- ), an American chemist
Plant Tissue Grows “In Glass”
In the mid-1800’s, biologists began pondering whether a cell isolated
from a multicellular organism could live separately if it were
provided with the proper environment. In 1902, with this question in
mind, the German plant physiologist Gottlieb Haberlandt attempted
to culture (grow) isolated plant cells under sterile conditions on an artificial
growth medium. Although his cultured cells never underwent
cell division under these “in vitro” (in glass) conditions, Haberlandt
is credited with originating the concept of cell culture.
Subsequently, scientists attempted to culture plant tissues and
organs rather than individual cells and tried to determine the medium
components necessary for the growth of plant tissue in vitro.
In 1934, Philip White grew the first organ culture, using tomato
roots. The discovery of plant hormones, which are compounds that
regulate growth and development, was crucial to the successful culture
of plant tissues; in 1939, Roger Gautheret, P. Nobécourt, and
White independently reported the successful culture of plant callus
tissue. “Callus” is an irregular mass of dividing cells that often results
from the wounding of plant tissue. Plant scientists were fascinated
by the perpetual growth of such tissue in culture and spent
years establishing optimal growth conditions and exploring the nutritional
and hormonal requirements of plant tissue.
Plants by the Millions
A lull in botanical research occurred during World War II, but
immediately afterward there was a resurgence of interest in applying
tissue culture techniques to plant research. Georges Morel, a plant physiologist at the National Institute for Agronomic Research
in France, was one of many scientists during this time who
had become interested in the formation of tumors in plants as well
as in studying various pathogens such as fungi and viruses that
cause plant disease.
To further these studies, Morel adapted existing techniques in order
to grow tissue from a wider variety of plant types in culture, and
he continued to try to identify factors that affected the normal
growth and development of plants. Morel was successful in culturing
tissue from ferns and was the first to culture monocot plants.
Monocots have certain features that distinguish them fromthe other
classes of seed-bearing plants, especially with respect to seed structure.
More important, the monocots include the economically important
species of grasses (the major plants of range and pasture)
and cereals.
For these cultures, Morel utilized a small piece of the growing tip
of a plant shoot (the shoot apex) as the starting tissue material. This
tissue was placed in a glass tube, supplied with a medium containing
specific nutrients, vitamins, and plant hormones, and allowed
to grow in the light. Under these conditions, the apex tissue grew
roots and buds and eventually developed into a complete plant.
Morel was able to generate whole plants from pieces of the shoot
apex that were only 100 to 250 micrometers in length.
Morel also investigated the growth of parasites such as fungi and
viruses in dual culture with host-plant tissue. Using results from
these studies and culture techniques that he had mastered, Morel
and his colleague Claude Martin regenerated virus-free plants from
tissue that had been taken from virally infected plants. Tissues from
certain tropical species, dahlias, and potato plants were used for the
original experiments, but after Morel adapted the methods for the
generation of virus-free orchids, plants that had previously been
difficult to propagate by any means, the true significance of his
work was recognized.
Morel was the first to recognize the potential of the in vitro culture
methods for the mass propagation of plants. He estimated that several
million plants could be obtained in one year from a single small
piece of shoot-apex tissue. Plants generated in this manner were
clonal (genetically identical organisms prepared from a single plant).With other methods of plant propagation, there is often a great variation
in the traits of the plants produced, but as a result of Morel’s
ideas, breeders could select for some desirable trait in a particular
plant and then produce multiple clonal plants, all of which expressed
the desired trait. The methodology also allowed for the production of
virus-free plant material, which minimized both the spread of potential
pathogens during shipping and losses caused by disease.
Consequences
Variations on Morel’s methods are used to propagate plants used
for human food consumption; plants that are sources of fiber, oil,
and livestock feed; forest trees; and plants used in landscaping and
in the floral industry. In vitro stocks are preserved under deepfreeze
conditions, and disease-free plants can be proliferated quickly
at any time of the year after shipping or storage.
The in vitro multiplication of plants has been especially useful
for species such as coconut and certain palms that cannot be propagated
by other methods, such as by sowing seeds or grafting, and
has also become important in the preservation and propagation of rare plant species that might otherwise have become extinct. Many
of these plants are sources of pharmaceuticals, oils, fragrances, and
other valuable products.
The capability of regenerating plants from tissue culture has also
been crucial in basic scientific research. Plant cells grown in culture
can be studied more easily than can intact plants, and scientists have
gained an in-depth understanding of plant physiology and biochemistry
by using this method. This information and the methods
of Morel and others have made possible the genetic engineering and
propagation of crop plants that are resistant to disease or disastrous
environmental conditions such as drought and freezing. In vitro
techniques have truly revolutionized agriculture.